韩文元
博士,教授,博士生导师
邮箱:hanwenyuan@mail.hzau.edu.cn
主讲课程:合成生物学,代谢工程
研究生招生专业:微生物学,合成生物学与系统生物工程
学术兼职:担任Frontiers in Genome Editing的Associate editor
教育经历
2004.09-2011.07,山东大学,理学学士/硕士
2011.09-2015.05,丹麦哥本哈根大学,理学博士
主要职历
2015.08-2017.07,丹麦哥本哈根大学,博士后
2017.07-2018.03,丹麦哥本哈根大学,助理教授
2018.09-现在,bwin必赢,教授
科研成果
科研方向
细菌和古菌无时无刻不面临着病毒和质粒等外源遗传物质的入侵,为了应对这些威胁,它们进化出了各种各样的免疫系统。其中,获得性免疫系统具备序列特异性的核酸识别、切割功能,例如CRISPR-Cas9,从而被广泛应用于基因编辑、基因表达调控、核酸检测等领域。本课题组的研究方向包括(1)解析微生物免疫系统(包括CRISPR-Cas和argonaute等)应对病毒和质粒入侵的机制;(2)开发基于微生物免疫系统的应用技术。
欢迎对微生物免疫感兴趣的本科生、研究生加盟本课题组学习或继续深造。同时,本实验室招聘科研助理,博士后若干名,欢迎有志于科学研究的青年英才来信交流(hanwenyuan@mail.hzau.edu.cn)
主持科研项目
1. 国家自然科学基金面上项目,冰岛硫化叶菌Argonaute系统介导流产感染的分子机制,2023/01-2026/12,54万元
2. 华中农业大学自主科技创新基金,原核生物Argonaute免疫机制的研究,2020/06-2023/05,30万元
3. 国家重点研发计划,基于P450 调控的自由基反应催化合成氮、硫杂环分子(子任务),2020/01-2024/12,61万元
4. 国家自然科学基金面上项目,古菌B家族DNA聚合酶执行跨损伤合成分子机制的研究,2020/01-2023/12,58万元
5. 华中农业大学校高层次人才启动经费,V-U亚型CRISPR系统以及短型Argonaute系统工作机制及应用的研究,2019/01-2023/12,200万元
代表性学术论文
1. Zhifeng Zeng, Yu Chen, Rafael Pinilla-Redondo, Shiraz A. Shah, Fen Zhao, Chen Wang, Zeyu Hu, Chang Wu, Changyi Zhang, Rachel J. Whitaker, Qunxin She, Wenyuan Han*. A short prokaryotic Argonaute activates membrane effector to confer antiviral defense. Cell Host & Microbe 2022 Jul 13;30(7):930-943.e6. (通讯作者)
2. Shunhang Liu, Xichen Rao, Ruiliang Zhao, Wenyuan Han*. The trans DNA cleavage activity of Cas12a provides no detectable immunity against plasmid or phage. Front Genome Ed. 2022 Jul 26;4:929929. (通讯作者)
3. Ruiliang Zhao, Yang Yang, Fan Zheng, Zhifeng Zeng, Wenqian Feng, Xuexia Jin, Jiayi Wang, Ke Yang, Yun Xiang Liang, Qunxin She, Wenyuan Han*. A membrane-associated DHH-DHHA1 nuclease degrades type III CRISPR second messenger. Cell Rep. 2020 Sep 15;32(11):108133. (通讯作者)
4. Tong Guo, Fan Zheng, Zhifeng Zeng, Yang Yang, Qi Li, Qunxin She, Wenyuan Han*. Cmr3 regulates the suppression on cyclic oligoadenylate synthesis by tag complementarity in a Type III-B CRISPR-Cas system. RNA Biol. 2019 Oct;16(10):1513-1520. (通讯作者)
5. Wenyuan Han, Stefano Stella, Yan Zhang, Tong Guo, Karolina Sulek, Li Peng-Lundgren, Guillermo Montoya, Qunxin She*. A Type III-B Cmr effector complex catalyzes the synthesis of cyclic oligoadenylate second messengers by cooperative substrate binding. Nucleic Acids Res. 2018 Nov 2;46(19):10319-10330.
6. Tong Guo, Wenyuan Han*, Qunxin She*. Tolerance of Sulfolobus SMV1 virus to the immunity of I-A and III-B CRISPR-Cas systems in Sulfolobus islandicus. RNA Biol. 2019 Apr;16(4):549-556. (共同通讯作者)
7. Wenyuan Han, Saifu Pan, Blanca Lopez-Mendez, Guillermo Montoya and Qunxin She. Allosteric regulation of Csx1, a type IIIB-associated CARF domain ribonuclease by RNAs carrying a tetraadenylate tail. Nucleic Acids Res. 2017 Oct 13;45(18):10740-10750.
8. Wenyuan Han#, Yingjun Li#, Ling Deng, Mingxia Feng, Wenfang Peng, Søren Hallstrøm, Jing Zhang, Nan Peng, Yun Xiang Liang, Malcolm F. White and Qunxin She. A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction. Nucleic Acids Res. 2017 Feb 28;45(4):1983-1993. (共同第一作者)
9. Wenyuan Han, Yanqun Xu, Xu Feng, Yun Xiang Liang, Li Huang, Yulong Shen and Qunxin She. NQO-induced DNA-less cell formation is associated with chromatin protein degradation and dependent on A0A1-ATPase in Sulfolobus. Front Microbiol. 2017 Aug 14;8:1480.
10. Wenyuan Han, Xu Feng and Qunxin She. Reverse gyrase functions in genome integrity maintenance by protecting DNA breaks in vivo. Int. J. Mol. Sci. 2017 Jun 22;18(7).
11. Wenyuan Han and Qunxin She. CRISPR history: discovery, characterization, application and prosperity. Prog Mol Biol Transl Sci. 2017;152:1-21.
12. Wenyuan Han, Yulong Shen and Qunxin She. Nanobiomotors of archaeal DNA repair machineries: current research status and application potential. Cell Biosci. 2014 Jun 25;4:32.
13. Pengjuan Liang#, Wenyuan Han#, Qihong Huang, Yanze Li, Jinfeng Ni, Qunxin She and Yulong Shen*. Knockouts of RecA-like proteins RadC1 and RadC2 have distinct responses to DNA damage agents in Sulfolobus islandicus. J Genet Genomics. 2013 Oct 20;40(10):533-42. (共同第一作者)